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KMID : 0624620080410040338
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BMB Reports
2008 Volume.41 No. 4 p.338 ~ p.343
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Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses
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Pulla Rama Krishna
Kim Yu-Jin
Kim Myung-Kyum
Senthil Kalai Selvi
In Jun-Gyo
Yang Deok-Chun
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Abstract
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Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration. [BMB reports 2008; 41(4): 338-343]
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KEYWORD
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Abiotic stress, Codonopsis lanceolata, Dehydrin (DHN), Semi-quantitative RT-PCR
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